The Capsid Protein of Turnip Crinkle Virus Overcomes Two Separate Defense Barriers To Facilitate Systemic Movement of the Virus in Arabidopsis'V
نویسندگان
چکیده
Depmtment of Plant Pathology, Ohio Af51icultural Research and Development Center, Ohio State University, 1680 Madison Ave., Wooster, Ohio 44691 ; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900; Com and Soybean Virns Unit, USDA-ARS, 1680 Madison Ave., Wooster, Ohio 44691 ; and Department of Cell Biology and Molecular Genetics, University of Mmyland, College Park, Maryland 20742
منابع مشابه
The capsid protein of Turnip crinkle virus overcomes two separate defense barriers to facilitate systemic movement of the virus in Arabidopsis.
The capsid protein (CP) of Turnip crinkle virus (TCV) is a multifunctional protein needed for virus assembly, suppression of RNA silencing-based antiviral defense, and long-distance movement in infected plants. In this report, we have examined genetic requirements for the different functions of TCV CP and evaluated the interdependence of these functions. A series of TCV mutants containing alter...
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Transient expression of proteins in plants has become a choice to facilitate recombinant protein production with its fast and easy application. On the other hand, host defensive mechanisms have been reported to reduce the efficiency of transient expression in plants. Hence, this study was designed to evaluate the effect of cap analog and Potato virus A helper component proteinase (PVA HC-Pro) o...
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The Arabidopsis flowering locus T (FT) gene encodes the mobile florigen essential for floral induction. While movement of the FT protein has been shown to occur within plants, systemic spread of FT mRNA remains to be unequivocally demonstrated. Utilizing novel RNA mobility assay vectors based on two distinct movement-defective viruses, Potato virus X and Turnip crinkle virus, and an agroinfiltr...
متن کاملHRT gene function requires interaction between a NAC protein and viral capsid protein to confer resistance to turnip crinkle virus.
An Arabidopsis protein was found to interact specifically with the capsid protein (CP) of turnip crinkle virus (TCV) through yeast two-hybrid screening. This protein, designated TIP (for TCV-interacting protein), was found to be a member of the recently recognized NAC family of proteins. NAC proteins have been implicated in the regulation of development of plant embryos and flowers. TIP alone w...
متن کاملThe coat protein of turnip crinkle virus suppresses posttranscriptional gene silencing at an early initiation step.
Posttranscriptional gene silencing (PTGS), or RNA silencing, is a sequence-specific RNA degradation process that targets foreign RNA, including viral and transposon RNA for destruction. Several RNA plant viruses have been shown to encode suppressors of PTGS in order to survive this host defense. We report here that the coat protein (CP) of Turnip crinkle virus (TCV) strongly suppresses PTGS. Th...
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تاریخ انتشار 2010